Stephen Rader's Publications

27. Wong, D.K., Grisdale, C.J., Slat, V.A., Rader, S.D., Fast, N.M. The evolution of pre-mRNA splicing and its machinery revealed by reduced extremophilic red algae. Journal of Eukaryotic Microbiology, 2022. https://doi.org/10.1111/jeu.12927. (PMID: 35662328)  PDF

26. Schärfen, L., Zigackova, D., Reimer, K.A., Stark, M.R., Slat, V.A., Francoeur, N.J., Wells, M.L., Zhou, L., Blackshear, P.J., Neugebauer, K.M., Rader, S.D.. Identification of Alternative Polyadenylation in Cyanidioschyzon merolae Through Long-Read Sequencing of mRNA. Frontiers in Genetics, 2022. https://doi.org/10.3389/fgene.2021.818697. (PMID: 35154260)  PDF

25. Wong, D.K., Stark, M.R., Rader, S.D., Fast, N.M. Characterization of Pre-mRNA Splicing and Spliceosomal Machinery in Porphyridium purpureum and Evolutionary Implications for Red Algae. Journal of Eukaryotic Microbiology, 2021. https://doi.org/10.1111/jeu.12844. (PMID: 33569840)  PDF

24. Bashir, S., Banday, M., Qadri, O., Bashir, A., Hilal, N., Fatima, N., Rader, S.D., Fazili, K.M. The molecular mechanism and functional diversity of UPR signaling sensor IRE1. Life Sciences, 2020, 265:118740. https://doi.org/10.1016/j.lfs.2020.118740. (PMID: 27928013)  PDF

23. Lang, I., Bashir, S., Lorenz, M., Rader, S.D., Weber, G. Exploiting the potential of Cyanidiales as a valuable resource for biotechnological applications. Applied Phycology, 2020, DOI: 10.1080/26388081.2020.1765702.  PDF

22. Garside, E.L., Whelan, T.A., Stark, M.R., Rader, S.D., Fast, N.M., Macmillan, A.M. Prp8 in a Reduced Spliceosome Lacks a Conserved Toggle that Correlates with Splicing Complexity across Diverse Taxa. Journal of Molecular Biology, 2019, 431:2543-2553. (PMID: 31078556)  PDF

21. Reimer, K.A., Stark, M.R., Aguilar, L.-C., Stark, S.R., Burke, R.D., Moore, J., Fahlman, R.P., Yip, C.K., Kuroiwa, H., Oeffinger, M., Rader, S.D. The sole LSm complex in Cyanidioschyzon merolae associates with pre-mRNA splicing and mRNA degradation factors. RNA, 2017, 23:952-967. (PMID: 28325844)  PDF

20. Black, C.S., Garside, E.L., MacMillan, A.M., Rader, S.D. Conserved structure of Snu13 from the highly reduced spliceosome of Cyanidioschyzon merolae. Protein Science, 2016, 25(4):911-916. (PMID: 26833716)  PDF

19. Hudson, A.J., Stark, M.R., Fast, N.M., Russel, A.G., Rader, S.D. Splicing diversity revealed by reduced spliceosomes in Cyanidioschyzon merolae and other organisms. RNA Biology, 2015 DOI:10.1080/15476286.2015.1094602. (PMID: 26400738).  PDF

18. Stark, M.R., Dunn, E.A., Dunn, W.S.C., Grisdale, C., Daniele, A.R., Halstead, M.R.G., Fast, N.M., Rader, S.D. Dramatically reduced spliceosome in Cyanidioschyzon merolae. PNAS, 2015, 112:E1191-E1200. (PMID: 25733880).  PDF

17. Dunn, E.A., Rader, S.D. Pre-mRNA splicing and the spliceosome: assembly, catalysis, and fidelity. Fungal RNA Biology. Sesma, A. and von der Haar, T. (eds) 2014, Springer-Verlag, Heidelberg, pp 27-57.  PDF

16. Stark, M.R., Rader, S.D. Complementation of U4 snRNA in S. cerevisiae splicing extracts for biochemical studies of snRNP assembly and function. Spliceosomal pre-mRNA Splicing: Methods and Protocols, Methods in Molecular Biology Series. K.J. Hertel ed. 2014, Humana Press, New York, 1126, pp 193-204.  PDF

15. Stark, M.R., Rader, S.D. Efficient splinted ligation of synthetic RNA using RNA ligase. Spliceosomal pre-mRNA Splicing: Methods and Protocols, Methods in Molecular Biology Series. K.J. Hertel ed. 2014, Humana Press, New York, 1126, pp 137-49.  PDF

14. Dunn, E.A., Rader, S.D. Preparation of yeast whole cell splicing extract. Spliceosomal pre-mRNA Splicing: Methods and Protocols, Methods in Molecular Biology Series. K.J. Hertel ed. 2014, Humana Press, New York, 1126, pp 123-35.  PDF

13. Hayduk, A.J., Stark, M.R., Rader, S.D. In vitro reconstitution of yeast splicing with U4 snRNA reveals multiple roles for the 3' stem-loop. RNA, 2012, 18:1075-1090. (PMID: 22411955)  PDF

12. Dunn, E.A., Rader, S.D. Secondary structure of U6 small nuclear RNA: implications for spliceosome assembly. Biochemical Society Transactions, 2010, 38:1099-1104. (PMID: 20659011)  PDF

11. Kim, S., Gorrell, A., Rader, S.D., and C.H. Lee. Endoribonuclease activity of human APE1 revealed by a real-time fluorometric assay. Analytical Biochemistry, 2009, 398:69-75. (PMID: 19932678)  PDF

10. Aukema, K.G., Chohan, K.K., Plourde, G.L., Reimer, K.B., and S.D. Rader. Small molecule inhibitors of yeast pre-mRNA splicing. ACS Chemical Biology, 2009, 4(9):759-768. (PMID: 19634919)  PDF

9. Thompson, M.D., Aukema, K.G., O'Bryan, D.M., Rader, S.D., and B.W. Murray. Plasmid sonication improves sequencing efficiency and quality in the Beckman-Coulter CEQTM8000. BioTechniques, 2008, 45(3):327-329. (PMID: 18778257)  PDF

8. Stark, M.R., Pleiss, J.A., Deras, M., Scaringe, S.A., and S.D. Rader. An RNA ligase-mediated method for the efficient creation of large, synthetic RNAs. RNA, 2006, 12: 1-6. (PMID: 16983143)  PDF

7. Stanek, D., Rader, S.D., Klingauf, M., and K.M. Neugebauer. Targeting of U4/U6 snRNP Assembly Factor SART3/p110 to Cajal Bodies. Journal of Cell Biology, 2003, 160: 505-516. (PMID: 12578909)  PDF

6. Rader, S.D., and C. Guthrie. An Lsm-Interaction Motif in Prp24 Required for Efficient U4/U6 Formation. RNA, 2002, 8: 1378-1392. (PMID: 12458792)  PDF 

5. Sauter, N.K., Mau, T., Rader, S.D., and D.A. Agard. Structure of the Complex Between Alpha-Lytic Protease and Its Pro Region. Nature Structural Biology, 1998, 5(11): 945-950. (PMID: 9808037)  PDF 

4. Rader, S.D., and D.A. Agard. α-Lytic Protease. Handbook of Proteolytic Enzymes, A. J. Barrett, N.D. Rawlings, and J.F. Woessner eds, 1998, Academic Press, pp 251-257. 

3. Rader, S.D., and D.A. Agard. Conformational Substates in Enzyme Mechanism: The 120 K Structure of Alpha-Lytic Protease at 1.5 Å Resolution. Protein Science, 1997, 6(7): 1375-1386. (PMID: 9232638)  PDF

2. Sohl, J.L., Shiau, A.K., Rader, S.D., Wilk, B.J., and D.A. Agard. Inhibition of Alpha-Lytic Protease by Pro Region C-terminal Steric Occlusion of the Active Site. Biochemistry, 1997, 36(13): 3894-3902. (PMID: 9092819)  PDF

1. Gregoret, L., Rader, S.D., Fletterick, R., and F. Cohen. Hydrogen Bonds Involving Sulfur Atoms in Proteins. Proteins, 1991, 9(2): 99-107. (PMID: 1755867)  PDF

Ice Sculpture at Lake Louise