Stephen Rader's Publications

18. Stark, M.R., Dunn, E.A., Dunn, W.S.C., Grisdale, C., Daniele, A.R., Halstead, M.R.G., Fast, N.M., Rader, S.D. Dramatically reduced spliceosome in Cyanidioschyzon merolae. PNAS, 2015, EE.  PDF

17. Dunn, E.A., Rader, S.D. Pre-mRNA splicing and the spliceosome: assembly, catalysis, and fidelity. Fungal RNA Biology. Sesma, A. and von der Haar, T. (eds), Springer-Verlag, Heidelberg, pp 27-57.  PDF

16. Stark, M.R., Rader, S.D. Complementation of U4 snRNA in S. cerevisiae splicing extracts for biochemical studies of snRNP assembly and function. Spliceosomal pre-mRNA Splicing: Methods and Protocols, Methods in Molecular Biology Series. K.J. Hertel ed. 2014, Humana Press, New York, 1126, pp 193-204.  PDF

15. Stark, M.R., Rader, S.D. Efficient splinted ligation of synthetic RNA using RNA ligase. Spliceosomal pre-mRNA Splicing: Methods and Protocols, Methods in Molecular Biology Series. K.J. Hertel ed. 2014, Humana Press, New York, 1126, pp 137-49.  PDF

14. Dunn, E.A., Rader, S.D. Preparation of yeast whole cell splicing extract. Spliceosomal pre-mRNA Splicing: Methods and Protocols, Methods in Molecular Biology Series. K.J. Hertel ed. 2014, Humana Press, New York, 1126, pp 123-35.  PDF

13. Hayduk, A.J., Stark, M.R., Rader, S.D. In vitro reconstitution of yeast splicing with U4 snRNA reveals multiple roles for the 3' stem-loop. RNA, 2012, 18:1075-1090.  PDF

12. Dunn, E.A., Rader, S.D. Secondary structure of U6 small nuclear RNA: implications for spliceosome assembly. Biochemical Society Transactions, 2010, 38:1099-1104.  PDF

11. Kim, S., Gorrell, A., Rader, S.D., and C.H. Lee. Endoribonuclease activity of human APE1 revealed by a real-time fluorometric assay. Analytical Biochemistry, 2009, 398:69-75.  PDF

10. Aukema, K.G., Chohan, K.K., Plourde, G.L., Reimer, K.B., and S.D. Rader. Small molecule inhibitors of yeast pre-mRNA splicing. ACS Chemical Biology, 2009, 4(9):759-768.  PDF

9. Thompson, M.D., Aukema, K.G., O'Bryan, D.M., Rader, S.D., and B.W. Murray. Plasmid sonication improves sequencing efficiency and quality in the Beckman-Coulter CEQTM8000. BioTechniques, 2008, 45(3):327-329.  PDF

8. Stark, M.R., Pleiss, J.A., Deras, M., Scaringe, S.A., and S.D. Rader. An RNA ligase-mediated method for the efficient creation of large, synthetic RNAs. RNA, 2006, 12: 1-6.  PDF

7. Stanek, D., Rader, S.D., Klingauf, M., and K.M. Neugebauer. Targeting of U4/U6 snRNP Assembly Factor SART3/p110 to Cajal Bodies. Journal of Cell Biology, 2003, 160: 505-516.  PDF

6. Rader, S.D., and C. Guthrie. An Lsm-Interaction Motif in Prp24 Required for Efficient U4/U6 Formation. RNA, 2002, 8: 1378-1392.  PDF 

5. Sauter, N.K., Mau, T., Rader, S.D., and D.A. Agard. Structure of the Complex Between Alpha-Lytic Protease and Its Pro Region. Nature Structural Biology, 1998, 5(11): 945-950. PDF 

4. Rader, S.D., and D.A. Agard. α-Lytic Protease. Handbook of Proteolytic Enzymes, A. J. Barrett, N.D. Rawlings, and J.F. Woessner eds, 1998, Academic Press, pp 251-257. 

3. Rader, S.D., and D.A. Agard. Conformational Substates in Enzyme Mechanism: The 120 K Structure of Alpha-Lytic Protease at 1.5 Å Resolution. Protein Science, 1997, 6(7): 1375-1386. PDF

2. Sohl, J.L., Shiau, A.K., Rader, S.D., Wilk, B.J., and D.A. Agard. Inhibition of Alpha-Lytic Protease by Pro Region C-terminal Steric Occlusion of the Active Site. Biochemistry, 1997, 36(13): 3894-3902. PDF

1. Gregoret, L., Rader, S.D., Fletterick, R., and F. Cohen. Hydrogen Bonds Involving Sulfur Atoms in Proteins. Proteins, 1991, 9(2): 99-107. PDF

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